Composite

Part:BBa_K2039002:Design

Designed by: Coline Sivelle   Group: iGEM16_Paris_Saclay   (2016-10-13)


Dimerization system FRB/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 716
    Illegal NheI site found at 739
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1255
    Illegal BamHI site found at 361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 866
    Illegal AgeI site found at 1040
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optomization for E. coli


Source

Composite part

References